Hydrodynamic chromatography (HDC). Mainly on size measurement of latex particle.

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fraction...

Particle Size of Latex Beads Dictates IL-1β Production Mechanism

Macrophages (Mϕ) are well documented to produce IL-1β through various signaling pathways in response to small particles such as silica, asbestos and urea crystals, in the presence of lipopolysaccharide (LPS). However, it has not been clear to what extent particle size affects the response. To investigate this point, we stimulated bone marrow-derived macrophages (BMDM) with size-defined latex be...

Measurement of Albuminuria with Size-exclusion Chromatography

Hydrodynamic Chromatography

Table 1, which also includes relative molecular mass data obtained from sequencing the solutes. It is seen that fairly accurate values for relative molecular mass can be obtained for the larger molecules, which can be extremely useful when dealing with completely unknown biopolymers. The errors involved are signiRcantly greater for the materials of smaller relative molecular mass because the re...

Measurement of low-density lipoprotein particle size by high-performance gel-filtration chromatography.

We describe a new technique for measuring LDL size by high-performance gel-filtration chromatography (HPGC). LDL was subjected to chromatography, and the column effluent was monitored at 280 nm. The retention time of the LDL peak was used to calculate the LDL diameter. We compared the HPGC method with gradient gel electrophoresis (GGE) on 2-10% nondenaturing polyacrylamide gels. In a group of 6...